Journal: Journal of Cellular and Molecular Medicine

Article Title: A novel antisense lncRNA NT5E promotes progression by modulating the expression of SYNCRIP and predicts a poor prognosis in pancreatic cancer

doi: 10.1111/jcmm.15718

Figure Lengend Snippet: LncNT5E is up‐regulated in pancreatic cancer (PC) tissues and cell lines. A, The heat map from our previous lncRNA microarray reflected the differentially expressed lncRNAs in PC and normal tissues. T represents PC tissue, and N represents normal pancreatic tissue. ENST00000421594 indicates lncNT5E. B, Relative expression of lncNT5E in 45 paired PC and adjacent normal tissues. LncNT5E expression from all tissues was normalized to 18S expression (ΔCT) and then compared with adjacent normal tissues and converted to the fold change (2 −ΔΔCT ). C, Relative expression of lncNT5E in different cell lines. Data are shown as fold change (2 −ΔΔCT ). * P < .05, ** P < .01, *** P < .001

Article Snippet: We used microarray detection (H1602063, Arraystar Human LncRNA 8 × 60 k v3.0 1‐color) to study lncRNAs in three pairs of PC and adjacent normal tissues.

Techniques: Microarray, Expressing

Journal: American Journal of Translational Research

Article Title: Microarray analysis reveals a potential role of lncRNA expression in remote ischemic preconditioning in myocardial ischemia-reperfusion injury

doi:

Figure Lengend Snippet: LncRNA and mRNA expression profile comparison between the RIPC group and the sham group in both the human and mouse. The hierarchical clustering of all target value human lncRNAs (A) and mRNAs (B). The scatterplot was used for assessing human lncRNA (A) and mRNA (B) expression variation between the RIPC group and the sham group. The hierarchical clustering of all the target value mouse lncRNAs (C) and mRNAs (D). The scatterplot was used for assessing mouse lncRNA (C) and mRNA (D) expression variation between the RIPC group and the sham group. Green lines inside the scatterplot picture represent the fold change lines (the default FC value was given 2.0). LncRNAs and mRNAs above the top green line were detected as up-regulated and below the bottom green line were detected as down-regulated, both of them were indicated as FC value >2.0 in expression. For example, “red dots” stand for higher relative expression levels (A), and “green dots” indicate low relative expression levels (B).

Article Snippet: Microarray hybridization data of mouse and human Arraystar mouse LncRNA microarray v3.0 and arraystar human LncRNA microarray v3.0 were specially designed for the global profiling of mouse & human LncRNAs and protein-coding transcripts.

Techniques: Expressing, Comparison

Journal: American Journal of Translational Research

Article Title: Microarray analysis reveals a potential role of lncRNA expression in remote ischemic preconditioning in myocardial ischemia-reperfusion injury

doi:

Figure Lengend Snippet: qRT-PCR validation of differential expressed lncRNAs. 8 LncRNAs were confirmed by qRT-PCR analysis to display significant changes between RIPC group and sham group (A). The data was expressed as the mean ± SEM (*P<0.05, **P<0.01). (B) qRT-PCR patterns of 9 lncRNAs were found to be consistent with that of the microarray data. The competing binding site was predicted by ceRNA mechanism analysis (C) and interaction has been shown in conception map (D).

Article Snippet: Microarray hybridization data of mouse and human Arraystar mouse LncRNA microarray v3.0 and arraystar human LncRNA microarray v3.0 were specially designed for the global profiling of mouse & human LncRNAs and protein-coding transcripts.

Techniques: Quantitative RT-PCR, Biomarker Discovery, Microarray, Binding Assay